KMID : 0381120210430091011
|
|
Genes and Genomics 2021 Volume.43 No. 9 p.1011 ~ p.1021
|
|
Baicalin attenuates adriamycin-induced nephrotic syndrome by regulating fibrosis procession and inflammatory reaction
|
|
Tan Ning
Sun Chen-Xia Zhu Hui-Jun Li De-Yu Huang Sheng-Guang He Shou-Di
|
|
Abstract
|
|
|
Background: Baicalin has anti-inflammatory, antibacterial, blood platelet aggregation-inhibiting, free oxygen radical-clearing, and endotoxin-decreasing properties. However, its molecular mechanism involved in the treatment of Adriamycin-induced nephrotic syndrome (NS) is still unclear.
Objective: This study aimed to explore the effects of baicalin on Adriamycin-induced nephrotic syndrome (NS) and to characterize the genes involved in this progression.
Methods: We established Adriamycin-induced NS model in 32 rats and used six rats in Sham group. Urinary total protein content and creatinine serum were assessed as physiological indicators. H&E staining was used to observe the pathological changes. We determined gene expression profiles using transcriptome sequencing in the rat kidney tissues from Sham, Adriamycin, and Adriamycin?+?baicalin groups. KEGG was carried out to analyze the enriched pathways of differentially expressed genes among these groups.
Results: Baicalin treatment relieved renal injury in NS rats. Expression of 363 genes was significantly different between the Adriamycin and Adriamycin?+?baicalin M groups. Most of the differentially expressed genes were enriched in pathways involved in epithelial-mesenchymal transition (EMT), fibrosis, apoptosis, and inflammation.
Conclusions: Overall, these data suggest that Adriamycin-induced NS can be attenuated by baicalin through the suppression of fibrosis-related genes and inflammatory reactions. Baicalin is a potential drug candidate for the treatment of NS, and the identified genes represent potential therapeutic targets.
|
|
KEYWORD
|
|
Nephrotic syndrome, Baicalin, Transcriptome sequencing, Fibrosis, Inflammatory reaction
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|